ELISA Kits are quick, accurate and reliable tools to identify a specific antibody or antigen in samples. These kits have pre-coated plates with capture and detection antibodies, a variety of reagents and standards that go through fit-for-purpose validation to ensure accuracy, sensitivity, and reproducibility. The versatility of ELISA kits make them an ideal choice for both clinical and research applications, especially for screening of potential biomarkers prior to further in-depth scientific testing.
ELISA expert are designed with an organized workflow to alleviate the time and frustration of manual preparation. They also reduce the need for tedious pipetting by including lyophilized, serially diluted standards in additional strip wells to eliminate the need for sample dilution. In addition, the plate coating with capture antibody is optimized for maximum binding capacity and the assay reagents are pre-mixed for ease of use.
ELISA Kits, Antibodies, and the Latest Innovations in the Antibody Market
In a sandwich-based ELISA assay, first the captured protein is detected using a second detection antibody that recognizes a specific epitope on the targeted protein. This technique increases sensitivity as it only detects proteins that are bound by the target antibody.
Moreover, a matched antibody pair in the capture and detection antibodies ensures maximum binding and detection. These ELISA assays are often used for measuring protein expression and phosphorylation in cell lysates. These ELISAs can help researchers understand biological pathways and their impact on disease states, such as aging, cancer, inflammation and more. In addition, ELISAs can be easily scaled up or down for high-throughput clinical screening to early-phase research experiments. This flexibility makes ELISA an accessible on-ramp for many labs.